![]() Researcher may use this product for research use only no transfer or commercial use of this product is allowed. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund. NanoLuc® ORF TE Vector Limited Use Label LicenseīY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may use this product for research use only, no commercial use is allowed. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. When the donor and acceptor proteins interact, energy transfer occurs, resulting in increased NanoBRET™ signal.īY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. The HaloTag® fusions are labeled with the fluorescent HaloTag® NanoBRET™ 618 Ligand and serve as the energy acceptor. In these assays, the target protein fused to NanoLuc® luciferase or a subunit of NanoBiT® enzyme and serves as the bioluminescent BRET energy donor. NanoBRET™ technology is a proximity-based method dependent upon energy transfer from a luminescent donor target protein to a fluorescent acceptor. The HaloTag® fusion vectors are designed to be used as the fluorescent acceptor in NanoBRET™ assays that monitor interactions of a specific target protein along the UPS pathway. An emerging modality for small-molecule drug development is targeting proteins for degradation via the UPS with small-molecule degraders such as PROTACs or molecular glues. The majority of proteins and their abundance are regulated via the ubiquitin proteasome system (UPS), which uses ubiquitin conjugation to signal proteins that should be trafficked to the proteasome for degradation. Regulation of cellular protein homeostasis is critical for maintaining cell health and often altered by cellular treatments or disease states.
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